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     Quattro’s enable Instrumental Liquid-Liquid,Preparative Chromatography and Extraction.. 
      Mass Scales:- Laboratory Scale of Milligrams, up to Industrial Scale of Tonnes Per Annum

• Save ~50-90% process chromatography/extraction, solvent/column costs
• Ideal HPLC-MS pre-prep; no column induced degradation or adsorption
• Uniquely allows infinitely polar to infinitely non-polar prep in one run
• Contract laboratory and process method development services available
• Easily scale-up from micro-gram to your tonne plus process chromatography scale
• Orthogonal selectivity options compared to process chromatography by HPLC/Flash/SFC
• No solubility problems; massive range of solvents available
• IL / SFC / SFC-MS / MS / MS-MS / HPLC / HPLC-MS compatible
• High process chromatography mass loading (typically 5 to 40 g per 1 litre capacity)
• Inject up to 10+ % coil volume without excessive band spreading
• High pressure (1000psi+) + high “G” (80-150)(250)(350+) options
• Only manufacturers of both hydrodynamic & hydrostatic CCC
• New MULTI-COIL, 2D & 3D DUAL-FLOW CCC/CPC extraction options,

Process Chromatography. Be a World Leader with Quattro CPC/CCC (API’s, Cannabinoids etc.)
A selection of some of the published articles or lectures related to Quattro CCC™ use or related to Dr Leslie Brown regarding CCC/HPL-LC™ are shown below.

1) Sutherland I.A., Brown L., Forbes S., Games D.E., Hawes D., Hostettmann K., McKerrell W.H., Marston A., Wheatley P., Wood P., J. Liq.Chrom & Re. Technol., 21(3), 1998, 279

2) Brown L., Wheatley D., Whiteside R., Kidwell H., Games D. E., Pittcon USA, 1999, Paper 776

3) Sutherland I.A., Booth A. J., Brown L., Kemp B., Kidwell H., Games D. E., Graham A.S., Guillion G.G., Hawes D., Hates M., Janaway L., Lye G. J., Massey P., Preston C., Shering P., Shoulder T., Strawson C., Wood P., J. Liq.Chrom & Re. Technol., 24(11&12), 2001, 1533

4) Whiteside R., Games D. E., Strawson C., Graham A.S., Brown L., Liq.Chrom & Re. Technol., 24(11&12), 2001, 1801

5) Graham A.S., McConvey., Shering P., Liq.Chrom & Re. Technol., 24(11&12), 2001, 1811

6) Comprehensive Analytical Chemistry XXXVIII, A Berthod (Ed), Countercurrent Chromatography, Chapter 2, Berthod A, Brown L, Leitao G.G, Leitao. S.G. Elsevier, NL, 2002

7) Vidal S., Hayasaka, Y., Meudec, E., Cheyner, V., Skouroumounis, G., J. Agric. Food Chem. (2004), 52, 713-719

8) Leitao G.G., El-Adji S.S., Melo, W.A., Leitao, S.G., Brown, L., J. Liq. Chromatogr. Relat. Technol. (2005) 28, 2041-2051

9) Leitao G.G., Souza, P.A., Moraes, A.A., Brown, L., J. Liq. Chromatogr. Relat. Technol. (2005) 28, 2053-2060

10) Silva J.C.T., Jham G.N., Oliveira, R.D. L., Brown, L., J. Chromatogr. A. (2007), 1151, 203-210

11) Wangenaar F.I., Hochlowski, J.E., Pan, J.Y., Tu, N.P., Searle, P.A, J. Chromatogr. A.1216 (2009) 4154-4160.

12) Brown L., Luu T.A., Hill C., Flockhart I., Garret P-H., Margraff R., Skouroumounis G., Chromatography Today 2009, 2 (4), 16-19 (with agreement Journal, copy available on this website)

13) Brown L., Luu T.A. J.Sep.Sci. 2010,33 999-1033

14) Brown L., Luu T.A. International Labmate e magazine July 2010 (+ printed copy)(search Liquid-Liquid Chromatography on Google for free access)

15) For publications in Chinese Literature please contact us on

A new centrifugal counter-current chromatograph and its application
- Murayama W, Kobayashi T, Kosuge Y, et al. -
CHROMATOGRAPHY, 1982 239 643-649

High-speed preparative countercurrent chromatography (CCC) with a coil
planet centrifuge
- Ito Y, Sandlin J, Bowers WC -
J CHROMATOGRAPHY,1982 244 247 +

Separation and purification of strychnine from crude extract of Strychinos Nux Vomica L by high-speed counter-current chromatography
- Miao P. Cai DC. Xiang BR, et al
J LIQUID CHROMATOGRAPHY,1998 21(1/2 Spec.lssue on CCC)163-l70.

Purification of anti-HIV ligands from Larrea Tridentata by ph-zone-refining counter-current chromatography
- Ma Y, Qi L, Gnabre JN, et al-
J LIQUID CHROMATOGRAPHY,1998 21(1/2 Spec.Issue on CCC)171-l81

Separation of a newly identified contaminant from commercial 4,5,6,7- tetrachlorofluorescein by ph-zone-refining counter-current chromatography
- Weisz A, Andrzejewskl D, Highet RJ, . et al-
J LIQUID CHROMATOGRAPHY 1998 21(1/2 Spec.Issue on CCC)183-1.93

Use of ph-zone-refining counter-current chromatography to separate 2- and 6-nitro-4-chloro-3-methoxybenzoic acid
-Dudding T, Mekonnen B, Ito Y,
J LIQUID CHROMATOGRAPHY.1998 21( Spec.Issue on CCC)195-201

Separation of epigallocatechin gallate and gallocatechin gallate using multiple instruments connected in series
-Du QZ, Ke CQ, ItoY
J LIQUID CHROMATOCRAPHY,1998 21(1/2 Spec.Issue on CCC)203-208

Preparative separation and purification of kaempferol, isorhamnetin, and quercetin by high-speed counter-current chromatography
- Yang FQ, Zhang TY, Ito Y, et al. et al-
J LIQUID CHROMATOGRAPHY,1998 2l(1/2 Spec.Issue on CCC)209-216

Separation of choroflurenol-methyl and determination of octanol-water coefficient by counter-current chromatography
- Mandava NB, Ito Y, Ma Y -
J LIQUID CHROMATOGRAPHY1998 21(1/2 Spec on CCC)217-229

Original uses of the liquid nature of the stationary phase in CCC I. Extraction and separation of transition metal ions
Talabardon K, Gageau N, Mermet 3M, et al. -
J LIQUID CHROMATOGRAPHY,1998 21(1/2 Spec.lssue n c:cC)23l-250

Concentration of heavy metals by high-speed counter-current chromatography
- Kitazume F, Sato N, Ito Y -
J LIQUID CHROMATOGRAPHY 1998, 21(1/2 Spec.Issue on CCC)251-261.

Retention of the stationary phase in a coil planet centrifuge: effects of interfacial tension / density difference and viscosities of liquid phases
Fedotov PS Thiehaut D -
J LIQUID CHROMATOGRAPRY,1998 21(1/2 Spec.Issuc on CCC)39.-51.

Comparison of counter-current chromatography with flash chromatography
- Conway WD, Bachert EL, Sarlo AM, et al. -
J LIQUID CHROMATOGRAPHY,1998 21(1/2 Spec.Issue on CCC)53-63.

Application of high speed counter-current chromatography, electrospray ionization mass spectrometry (HSCCC/ESI-MS) in natural products chemistry
- Kong Z, Rinehart KL, Milberg RM, et al -
J LIQUID CHROMATOGRAPHY,1998 21(1/2 Spec.Issue on CCC)65-82

Isolation and purification of 3-oxo-delta 5-steroid isomerase from crude E.coli lysate by counter-current chromatography
-Qi L Ma Y, ItoY
J LIQUID CHROMATOGRAPHY,1998 21(1/2 Spec.Issue on CCC)83-92

Toroidal coil counter-current chromatography separation of Staphylococcus aureus enterotoxin A in food
- Rasooly A, Ito Y -
J LIQUID CHROMATOGRAPHY, 1998 21(1/2 Spec. Issue on CCC)93-1O2.

Isolation of less polar alkali-labile glycolipids of human brain by high-speed counter-current chromatography
- Matsuda K, Ma Y,, Barghout V. et al. -
J LIQUID CHROMATOGRAPHY,J998 21(1/2 Spec.tssue on CCC)103-llO.

Protein separation by cross-axis coil planet centrifuge with two different types of coiled columns
- Shinomyia K, Kabasawa Y, Ito Y -
J LIQUID CHROMATOGRAPHY,1998 21(1/2 Spec.Issue on CCC)l1l-120.

Polyethylene glycol-potassium phosphate aqueous two phase systems for Counter-current chromatography of proteins
- Shibusawa Y, Yamaguchi M, Ito Y
J LIQUID CHROI'IATOCRAPHY,1998 21(1/2 Spcc.Issue on CCC)121-133

Enantiomeric separation of commercial D,L-kynurenine with an aqueous two phase solvent system by cross-axis coil planet centrifuge
- Shinomiya K, Kahasawa Y, Ito Y -
J LIQUID CHROMATOGRAPHY,1998 21(1/2 Spec.Issue on CCC)135-141

Isolation of colistin A and B using high-speed counter-current chromatography
- Ikai Y, Oka H, Hayakawa J, et al. -
J LIQUID CJIROMATOGRAPHY,1998 21(1/2 Spec.Issue on CCC)143-155.

Recycling high-speed counter-current chromatography for separation of taxol and cephalomannine
-Du QZ, Ke CQ, Ito Y
J LIQUID CHRONATOGRAPHY,1998 21(1/2 Spec.Issuc on CCC)157-162.

Toroidal coil counter-current chromatography study of mass transfer rate of proteins in aqueous-aqueous polymer phase system
- Ito Y, Matsuda K, Ma Y, et al,
J CHROMATOGRAPHY A, 1998 802, 277-283

Effects of coriolis force on counter-current chromatography
- Ito Y, Ma Y -
J LIQUID CHROMATOGRAPHY,1998 21(1/2 Spec.Issue on CCC)l-l7

Influence of composition and some physico-chemical properties of two-phase liquid systems on the stationary phase retention to coil planet centrifuge
- Maryutina TA, Ignatova SN, Fedotov PS, et al.
J LIQUID CHROMATOCRAPHY,1998 21(1/2 Spec.Issue on CCC)l9-37
Pressure drop in centrifugal partition chromatography
o van Buel MJ, van der Wielen ,LAM, Luyben KCAM -
J CHROMATOGRAPHY A, 1997 773 1-12.

Modelling gradient elution in centrifugal partition chromatography
- van Buel MJ, van der Wielen LAM, Luyben KCAM -
J CHROMATOGRAPHY A, 1997 773 13-12

Effect of foaming power and foam stability on continuous concentration with foam counter-current chromatography
- Oka H, lwaya M, Harada K, et ;sl. -
J CHROMATOGRAPHY A, 1997 791(1-2)53-64

Purification of lactic acid dehydrogenase from bovine heart crude extract by counter-current chromatography
- Shibusawa Y, Eriguchi Y, Ito Y -
J CHROMATOGRAPHY B,1997 696 25-31.

Lipoproteins: comparison of different separation strategies. Review
- Shibusawa Y -
J CHROMATOGRAPHY B,1997 699(1-2)419-437

Separation of taxol and cephalomannine by counter-current chromatography
- Chiou FY, Kan P, Chu TM, et al. -

High-speed counter-current chromatography: a promising method for the separation of the Annonaceous accotogenins
- Duret P, Waechter AJ, Margraff R, et al. -

Enrichment and separation of holmium and erbium by high-speed counter-current
- Akiba K, Hashimoto H, Nakamura S, et al. -

A new approach to continuous counter-current protein chromatography; direct purification of malate dehydrogenase from a Saccharomyces cerevisiae homogenate as a model system
- Owen RO, Mc Creath GE, Chase HA

Application of high-speed counter-current chromatography to the separation of coumarin and related compounds
- Shibusawa Y, Hagiwara Y, Chao Z, et al.
J CHROMATOGRAPHY A, 1997 759 47-53.

Nuclear magnetic resonance monitoring of centrifugal partition chromatography in ph-zone-refining mode
- Spraul M, Braumann U, Renault JH, e al, -
J CHROMATOGRAPHY A, 1997 766 255-260

Separation of samarium, gadolinum, terbium, gysprosium, erbium and ytterbium by high-speed counter-current chromatography with organophosphate ester
- Ma Z, Zhang L, Han S -
J CHROMATOGRAPHY A,1977 766 282-285

Preparative. separation of bitter acids from hop extracts by centrifugal partition chromatography
- Hermans-Lokkerbol ACJ, Hoek AC. Verpoorte R -
J CHROMATOGRAPHY A,1997 771 71-79.

Preparative separation by ph-zone-refining counter-current chromatography
-Ma Y, Ito Y
CHROMATOGRAPHY A, 1997 771 81-88.

Modelling gradient elution in centrifugal partition chromatography
- van Buel M van der Wielen LAM, Luyben KCAM -
J CHROMATOGRAPHY A, 1997 773 13-22, 12 ref.

Centrifugal partition chromatographic separation of tervalent lanthanides using acylpyrazolone extractants
- Ma C, Freiser H, Muralidharan S -ref.
ANAIXTICAL. CHEMISTRY, 1997 69 3835-2841.
Countercurrent chromatographic analysis of ovalbumin obtained from various sources using the cross-axis coil planet centrifuge
- Shinomiya K, Inokuchi C, Mishina M at al. -
J CHROMATOGAPHY A,1996 724(1-2)179-184

pH-zone refining counter-current chromatography of polar catecholamines
using di-(2-ethylhexyl)phosphoric acid as a ligand
- Ma Y, Sokolowski E , Ito Y-
J CHROMATOGRAP A, 1996 724(1-2)348-353

Preliminary design of a simulated counter-current chromatographic system for the separation of praziquantel enantiomers
- Lim BC, Ching CB -
J CHROMATOGRAPY A, 1996 734(2)247-258

ph-zone -refining countercurrent chromatography
- Ito Y, Ma Y
J CHROMATOGRAPHY A,1996 753 1-36.

Isolation of cardenolides from a Brazilian cultivar of Digitalis lanata by rotation locular counter-current chromatography
- Braga PC, Kreis W, de Oliveira AB -
J CHROMATOGRAPHY B, 687(2)287-291

Separation of wild-type lipopolysaccharides by centrifugal partition chromatography: isolation of smooth form species
- .Suda Y, Shiyama T, Yasukochi T, et al. -

Protein separation by improved cross-axis coil planet centrifuge with eccentric coil assemblies
- Shinomiya K, Muto M, Kabasawa Y, et a].. -

Purification of phosphatidylcholine with high content of DNA from squid Illex Argentinus by countercurrent chromatography
- Baudimant G, Maurice M, Landrein A, et al,

Application of perfluorocarbons in novel continuous counter-current protein chromatography
- Owen RO, mcCreath CE, Chase HA -
J MOLECULAR RECOGNITION,1996 9(5-6)575-584

High Speed Countercurrent Chromatography
Ito Y, Conway WD -
Chemical Analysis Series: No.132, John Wiley & Sons Inc - New York.

Measurement of partition coefficients in waterless biphasic liquid systems by countercurrent chromatography
- Berthod A, Allet AJ, Bully M -
ANALYTICAL CHEMISTRY, 1996 68(3)431-436

Centrifugal partition chromatographic separations of platinum group metals by complexation and ion pair formation
- Surakitbanham Y. Freiser H, Muralidharan S -
ANALTICAL CHEMISTRY,1996, 68, 3934-3938

Purification of mast cells with an improved non-synchronous flow-through coil planet centrifuge - Okada T, Metcalfe PD, Ito Y -

Identification and characterization of various differentiative growth plate chondrocytes from porcine by countercurrent centrifugal elutriation
- Lee KM, Fung KP, Leung PC, et al. -
J CELLULAR BIOCHgMISTRY,1996 60(4)508-520.

Preparative separation of phenolic compounds from Pica abies by high-speed counter -current chromatography
Slimestad R, Marston A, Hostettman K
J CHROMATOGRAPHY A,1996 719(2)438-443

Separation of ivermectin components by high-speed counter-current chromatography
- Oka H, Ikai Y, Hayakawa J, et a]..
J CHROMATOGRAPHY A, 1996 723 (1)61-68.
Chiral separation by high-speed countercurrent chromatography
-MaY, ItoY -
ANALYTICAL CHEMISTRY, 1995 67(1)3069-3074

Macrophage activation in response to S-form lipopolysaccharides (LPS) separated by centrifugal partition chromatography from wild-type LPS: effects of the polysacchride portion of LPS
- Suda Y, Kirikae T, Shiyama T, et al. -

Effective bioconversion with continuous product recovery using AOT/lecithin mixed reverse miceller systems and centrifugal partition chromatography as a novel bioreactor
- Yamada Y, Kuboi R, Komasawa I -

Rs-22A,B and C: a new macrolide antibiotics from Streptomyces violaceusniger. I.Taxonomy, fermentation, isolation and biological activities
- Ubukata M, Shiraishi N, Kobinata K, et al. -
ANTIBIOTICS, 995 48(4)289-292

Separation and purification of peptides by high-speed counter-current chromatography
- Knight M, Fagarasan MO, Takahashi K, et al. -
CHROMATOGRAPHY A,l995 702(1-2)207-2 14

Resolution of gram quantities of racemates by high-speed counter-current chromatography
- MaY, ItoY, FoucaultA -
CHROMATOGRAPHY, 1995 704(1)75-82

Purification of 25-hydroxy-cholecalciferol from irradiation of cholesta-5, 7 diol by high-speed countercurrent chromatography
-DuQH, ZhangTY, toY -
LIQUID CHROMATOGRAPHY, 1995 18(1)181-188

Semi-preparative separation of taraxeryl-acetate and coumarins from Artemesia dalailamae Kraschen by high speed countercurrent chromatography
- Yang FQ, Ou QY, Wu WL et al. -

Separation of yttrium from lanthanoid elements by centrifugal partition chromatography
- Nakamura S, Watanabe M, Akiba K -

An evaluation of the differential partitioning and separation of C-60 and C-70 fullerenes in a biphasic system using centrifugal partition chromatography (CPC)
- Gasper MP, Berthod A, Talabardon K, et al. -

Separation of two major anthocynins from champagne vintage by-products by gradient elution centrifugal partition chromatography
- RenaultJH, Thepenier P, Zeches Hanrot M.

Separation of bioactive quadri-terpenic acids from the fruit of Ligustrum lucidum ait by high-speed countercurrent chromatography
-DuQZ, XiongXP, ltoY -
LIQUID CHROMATOGRAPHY, 1995 18(10)1997-2004

Mutual separation of holmium, erbium, and yttrium by high-speed countercurrent chromatography
- Akiba K, Hashimoto H, Nakamura S, et al. -
LIQUID CHROMATOGRAPHY, l995 18(14)2723-2742

Isolation of antifungal and larvicidal constituents of Diplilophium buchanani by centrifugal partition chromatography
- Marston A, Hostettmann K, Msonthi JD -
NATURAL PRODUCTS, 1995 58(1)128-130

Countercurrent chromatography as an enzymatic reactor for organic synthesis
- Bousquet OR, Braun J, LeGoffic F -
TETRAHEDRON LETTERS, 995 36(45)8195-8196
Centrifugal partition chromatographic extraction of phenols and pesticides from water samples
- Liu Y, Lopez Avila V, Alcarez M, et al. -
ANALYTICAL CHEMISTRY, 1994 66(24)4483-4489

Acquisition of viable-like properties of sperm cells by adsorption of seminal plasma proteins revealed by centrifugal countercurrent distribution
- Pascual ML, Muino Blanco T, Cerbrian Perez JA, et al. -
BIOLOGY OF THE CELL I994 82(1)75 +

Aldecalmycin, a new antimicrobial antibiotic from Streptomyces. I. Taxonomy, fermentation, isolation, physico-chemical and biological properties
- Sawa R, Takahashi Y, Itoh 5, et al. -
ANTIBIOTICS TOKYO, 199447(11)1266-1272

Review. Counter current chromatography as a preparative tool - applications and perspectives
- Marston A, Hostettmann K -
CHROMATOGRAPHY A,1994 658 315-351. 165 refs

Separation of alkaloids by pH-zone-refining counter-current chromatography
- Ma Y, Ito Y, Sokolosky E, et al. -
CHROMATOGRAPHY, 1994 685(2)259-262

Purification of (-)-epigallocatechin from enzymatic hydrolysate of its gallate high-speed counter-current chromatography - Du 0, Li M, Cheng 0, et al. -
CHROMATOGRAPHY A, 1994 687(1)174-177

Preparative separation of naphthyltetrahydroisoquinoline alkaloids from Ancistrocladus korupensis by centrifugal
partition chromatography - Hallock YF, Dai J, Bokesch HR, et al. -
CHROMATOGRAPHY A, 1994 688(1-2)83-88

Centrifugal partition chromatography - Foucault AP (Ed) - Marcel Dekker Inc - New York, 1994, Modelling of centrifugal partition chromatography
- van Buel MJ, van der Wielen LAM, Meester R, et al. - pp 515-519 in: EC86: Proceedings of the 6th European Congress on Biotechnology,ed.L
Alberghina, L Frontali & P Sensi, Elsevier Science, 1994. 7 ref.
Delaminomycins, novel nonpeptide extracellular matrix receptor antagonist and a new class of potent immunomodulator. I. Taxonomy, fermentation, isolation and biological activity
- Ueno M, Amemiya M, lijima M, et al. -
ANTIBIOTICS, 46(5)719-727

Respinomycins AI,A2,B,C and D a novel group of anthracycline antibiotics. I. Taxonomy, fermentation, isolation and biological activities
- Ubukata M, Osada H, Kudo 1, et al. -
ANTIBIOTICS, 46(6)936-941

Studies on new cross-axis coil planet centrifuge for performing counter-current chromatography. I. Design of the apparatus, retention of the stationary phase, and efficiency in the separation of proteins with polymer phase systems
- MenetJM, Shinomiya K, Ito Y -
CHROMATOGRAPHY, 644(2)215-229. 14 ref.

Studies on new cross-axis coil planet centrifuge for performing counter-current chromatography. II. Path and acceleration of coils and comparison with type
coil planet centrifuge
- MenetJM, Ito Y -
CHROMATOGRAPHY,I993 644(2)231-238. 10 ref.

Studies on new cross-axis coil planet centrifuge for performing counter-current chromatography. Ill. Speculations on the hydrodynamic mechanism in stationary phase retention
- Menet JM, Shinomiya K, Ito Y -
CHROMATOGRAPHY, 1993 644(2)239-252. 8 ref.

Hydrodynamics of centrifugal partition chromatography
- Meester R, van Buel MJ, van der Wielen LAM, et al. -
pp 295-303 in: Proceedings of Precision Process Technology, ed. MPC Weijnen &
AAH Drinkenburg, Delft, 1993
Effector-assisted refolding of recombinant tissue plasminogen activator produced in Escherichia coli
- Grunfeld H, Patel A, Shatzman A, et al. -

Inactivation of proteins and other biological macromolecules during chromatographic methods of bioseparation
-SadanaA BIOSEPARATION, 1992 3(2-3)145-165

Quantitative structure-metabolism relationship analyses of MAO-mediated toxication of I -methyl-4-phenyl- I ,2,3,6-tetrahydropyridine and analogues
- Altomare C, Carrupt PA, Gaillard P, et al. -

Saponins with an unusual secoursene skeleton from Sesamum alatum THONN
- Potterat 0, Hostettmann K, Stoeckli-Evans H, et al. -
HELVETICA CHIMICAACTA,1992 75833-841. 20 ref.

3-butyl 5,6-dihydro-4H-isobenzofuran- I-one, a sensorial active phthalide in parsley roots
- Nitz 5, Spraul MH, Drawert F -

Benarthin: a new inhibitor of pyroglutamyl peptidase. I. Taxonomy, fermentation, isolation and biological activities
- Aoyagi 1, Hatsu M, Kojima F, et al. -
ANTI BIOTICS, 1992 45(7)1079-1083

Counter-current chromatography of lipoproteins with a polymer phase system using the cross-axis synchronous coil planet centrifuge
- Shibusawa Y, Ito Y, Okewaki K, et al. -
CHROMATOGRAPHY, 1992 596(1)118-122

Studies on an abnormally sharpened elution peak observed in counter-current chromatography
- Ito Y, Shibusawa Y, Fales HM, et al. -

High speed countercurrent chromatography separations of Taxol and related diterpenoids from Taxus baccata
- Vanhaelen-Fastre R, Diallo B, Jaziri M, et al. -
LIQUID CHROMATOGRAPHY, 5(4)697-706. 13 ref.

Automation of countercurrent chromatography (CCC) via personal computer
- Ernst UP, Hsu JT, Chou FE -
LIQUID CHROMATOGRAPHY, 15(15-16)2677-2690. 5 ref.

Importance of the parameter Vm/Vc in countercurrent chromatogrsphy. Tentative comparison between instrument designs
- Foucault AP, Bousquet 0, Le Goffic F -

Fundamental chromatographic parameters in countercurrent chromatography:
influence of the volume of stationary phase and the flow rate
- MenetJM, Rolet MC, Rosset R, et al. -
LIQUID CHROMATOGRAPHY, 15(15-16)2883-2908. 5 ref.

Anti-HIV and cytotoxic alkaloids from Buchenavia capitata
- Beutler JA, Cardellina JH, McMahon jB, et al. -
NATURAL PRODUCTS, 1992 55(2)207-213. 14 ref.

Alkaloids of Stipa robusta (Sleepygrass) infected with an Acremonium endophyte
- Petroski RJ, Powell RG, Clay K -
NATURAL TOXINS, I 84-88. 13 ref.

Xanthones and secoiridoid glucosides of Halenia campanulata
- Recio-Iglesias MC, Marston A, Hostettmann K -
PHYTOCHEMISTRY, 1992 31(4)1387-1389. 13 ref.

Three nitropropanoyl esters of glucose from Indigofera Iinnaei
- Majak 'N, Benn M, McEwan D, et al. -
PHYTOCHEMISTRY,I992 31(7)2393-2395. 16 ref.

New trichoverroids from Myrothecium verrucaria isolated by high speed countercurrent chromatography
- Jarvis BB, DeSilva I -
NATURAL PRODUCTS,I992 55(10)1441-1446. II ref.
Countercurrent chromatography
- Foucault AP -

Dunaimycins, a new complex of spiroketal 24-membered macrolides with immunosuppressive activity II Isolation and elucidation of structures
- Hochlowski JE, MuIlally MM, Brill GM, et al. -
jANTIBIOTICS,1991 44(12)13181330. 12 ref.

Recent advances in counter-current chromatography
- Ito Y -
CHROMATOGRAPHY,199 538(1)3-25

Solvent selection guide for counter-current chromatography
- Abbot TP, Klelman R -
CHROMATOGRAPHY,1991 538 109-118

Toroidal coil centrifugal partition chromatography, a method for measuring partition coefficients
- Tsai RS, eI-Tayar N, Testa B, et al. -
CHROMATOGRAPHY,1991 538(1)119-123. 14 ref.

Preparative purification of tetrabromotetrachlorofluorescein and phloxine B by centrifugal counter-current chromatography
- Weisz A, Langowski AJ, Meyers MB, et al. -
CHROMATOGRAPHY, 538(1)157-164

Improved high-speed counter-current chromatograph with three multilayer coils connected in series. II Separation of various biological samples with a semi- preparative column
-ItoY, OkaH, LeeYW -
CHROMATOGRAPHY, 498 169-178. 4 ref.

Semi-preparative purification of an endogenous ligand for brain serotonin-2 receptors by coil planet centrifuge counter-current chromatography
-ApudjA, ItoY -
CHROMATOGRAPHY,199I 538(1)177-185

Recent applications of counter-current chromatography to the isolation of bioactive natural products
- Kubo I -

Applications of high-speed counter-current chromatography for the separation and isolation of natural products
- Fischer N, Weinrich B, Nitz S, et al. -
CHROMATOGRAPHY, 538 193-202. 19 ref.

Isolation of bacitracins A & F by high-speed counter-current chromatography
- Harada KI, Ikai Y, Yamazaki Y, et al. -
CHROMATOGRAPHY, 538 203-2 12

Protein separation with aqueous-aqueous polymer systems by two types of counter-current chromatographs
- Shibusawa Y, Ito Y -
CHROMATOGRAPHY. 1991 550(1-2)69

Direct coupling of high-speed counter-current chromatography to thin-layer chromatography. Application to the separation of asiaticoside and madecassoside from Centella asiatica
- Diallo B, Vanhaelen-Fastre, Vanhaelen M -
CHROMATOGRAPHY,I99 558 446-450

Novel alkaloids from the tropical plant Ancistrocladus abbreviatus inhibit cell killing by HIV-I and HIV-2
- Manfredi -

Antineoplastic agents, 177. Isolation and structure of phyllanthostatin 6
- Petit GR, Schaufrlberger DE, Nieman RA, et al. -
NATURAL PRODUCTS,1990 53(6)1406-1413. 17 ref.

Determination of lipophilicity and hydrogen-bond acidity of bioactive sulphonyl containing compounds by reversed-phase HPLC and centrifugal partition chromatography and their application to structure-activity relations
- Altomare C, Tsai RS, el Tayar N, et at. -
PHARMACY & PHARMACOLOGY, 1991 43(3)191-197

The isolation of Eleutheroccus senticosus constituents by centrifugal partition chromatography and their quantitative determination by high performance liquid chromatography
- Slacanin I, Marston A, Hostettmann K, et al. -
PHYTOCHEMICAL ANALYSIS,199I 2 137-142. 23 ref.

Iridoid glucosides from Rogeria adenophylla
- Potterat 0, Saadou M, Hostettmann K -
PHYTOCHEMISTRY, 30(3)889-892. 17 ref.

Research on African medicinal plants - xxv+ - the (I R,2S) absolute configuration of nyasicoside, Its occurrence in Curculigo recurvata
- Chifundera K -
TETRAHEDRON, 1991 47(25)4369-4374. 9 ref.

Evaluation of analytical countercurrent chromatographs: high speed countercurrent chromatograph-4000 vs analytical toroidal coil centrifuge
- Oka H, Ikai Y, Kawamura N, et al. - LIQUID CHROMATOGRAPHY,I990 13 2309

Cl7 polyacetylene alcohols as the major constituents in roots of Petroselinum crispum Mill ssp.tuberrosum
- Nitz 5, Spraul MH, Drawert F -
AGRICULTURE & FOOD CHEMISTRY, 38 1445-144722 ref.
Centrifugal counter-current chromatography, a promising means of measuring partition coefficients
- Vallat P. El Tayar N, Testa B, et al. -
CHROMATOGRAPHY, 1990 504 411-419. 25 ref.

The application of high-speed countercurrent chromatography to the semipreparative separation of vincamine and vincine
- Zhou JY, Fang QC -

Sweet-tasting triterpene glycoside constituents of Abrus fruticulosus
- Fullas F, Choi YH, Kinghorn AD, et al. -
PLANTA MEDICA, 56332-333. 7 ref.
Phenolic constituents of licorice. II. Structures of licopyranocoumarin, licoarylcoumarin and glisoflavone, and inhibitory effects of licorice phenolics on xanthine oxidase
- Hatano T, Yasuhara T, Fukuda 1, et at. -

Accumulation of unique globo-series glycolipids in PC I 2h pheochromocytoma cells
- Arige T, Suzuki M, Yu RK, et al. -
BIOLOGICAL CHEMISTRY, 1989 264(3)1516-1521

Improved cross-axis synchronous flow-through cell planet centrifuge for performing counter-current chromatography. I Design of the apparatus and analysis of acceleration
- toY, Oka H, SlempJL -
CHROMATOGRAPHY,1989 463(2)305-3 16

Use of centrifugal partition chromatography for assessing partition coefficients in various solvent systems
- El Tayar N, Marston A, Bechalany A, et al. -
CHROMATOGRAPHY,1989 469 91-99. 34 ref.

Improved high-speed counter-current chromatograph with three multilayer coils connected in series. I. Design of the apparatus and performance of semipreparative columns in 2,4-dinitrophenyl amino acid separation
- Ito Y, Oka H, SlempJL -

Application of analytical high-speed countercurrent chromatography to the isolation of bioactive natural products
- Lee YW, Cook CE, Fang QC, et al. -
CHROMATOGRAPHY, 1989 477 434-438

Modifications to high speed counter-current chromatography for improved separation capability
- Slacanin I -
CHROMATOGRAPHY, 1989 482 234-239

Centrifugal counter-current partition chromatography with helical coil rotor. Simplified counter-current chromatography with a rotating face-seal
- Kim LS, han ZO, Li XN, et al. -
CHROMATOGRAPHY,I989 483 359-368

Comparing centrifugal countercurrent chromatography, nonaqueous reversed phase HPLC and Ag ion exchange HPLC for the separation and characterization of triterpene acetates- Abbott T, Peterson R, McAlpine J, et al. -
LIQUID CHROMATOGRAPHY,1989 12(2)2281-2301. 37 ref.

Plant antiviral agents VI: Isolation of antiviral phenolic glucosides from Populus cultivar Beaupre by droplet counter-current chromatography
- Van Hoof L Totte j, Corthout J, et al. -
NATURAL PRODUCTS, 1989 52(4)875-878

Benzylisoquinoline alkaloids from Anisocycla cymosa roots
- Kanyinda B, Diallo B, Vanhaelen-Fastra, et al. -
PLANTA MEDICA,l989 55 394-395. 16 ref.

Application of droplet counter-current chromatography to the isolation of vitamin Bl2
- Kurumaya K, Sakamoto T, Okada Y, et al. -
CHROMATOGRAPHY, 1988 435(1)235-240
Isolation of biologically active plant constituents by liquid chromatography
- Marston A, Potterat 0, Hostettmann K - CHROMATOGRAPHY, 1988 450(1)3-Il

Separation of natural products by centrifugal partition chromatography
- Marston A, Borel C, Hostettmann K - CHROMATOGRAPHY, 198845091-99
i6) 649073 Facsimile 0 (1656) 656099

Isolation of virginiamycin M I by droplet counter-current chromatography
- Saito K, Hone M, Hoshino Y, et al. - CHROMATOGRAPHY,1988 454 387-39 I

New high-speed countercurrent chromatography equipped with a pair of separation columns
-ItoY, ChouFE CHROMATOGRAPHY, 475 382 +

Analytical high-speed countercurrent chromatography
- Lee YV Ito Y, Fang OC, et al. - LIQUID CHROMATOGRAPHY, II 75-79

Separation and purification of macrolides using the Ito multi-layer horizontal coil planet centrifuge
- Chen RH, Hochlowski JE, McAlpine J8, et al. - LIQUID CHROMATOGRAPHY, II 191-201. 10 ref.

Large scale purification of Apocarotenoids from cochlospermum tinctorium by counter-current chromatography
- Diallo B, Vanhaelen M - LIQUID CHROMATOGRAPHY, 1988 11(1)227-231. 5 REF.

Separation of flavonoids in crude extract from sea buckthorn by countercurrent chromatography with two types of coil planet centrifuge
- Zhang TY, Hua X, Xiao R, et al. - LIQUID CHROMATOGRAPHY,I988 11(1)233-244. 10 ref.

Preparative separation of unsaturated fatty acids esters by centrifugal partition chromatography
- Murayama W, Kosuge Y, Nakaya N, et al. - LIQUID CHROMATOGRAPHY,I988 II 283-330

Centrifugal partition chromatography I. General features
- Berthod A, Armstrong DW - LIQUID CHROMATOGRAPHY, II 547-566

Centrifugal partition chromatography. II. Selectivity and efficiency
- Berthod A, Armstrong DW - LIQUID CHROMATOGRAPHY, II 567-583

Countercurrent chromatography for isolation of flavonol glycosides from ginkgo biloba leaves
- Vanhaelen M, Vanhaelen-Fastre P. - LIQUID CHROMATOGRAPHY, 11(14)2969-2975. 9 ref.

Native American food and medicinal plants. 8. Water-soluble constituents of Lomatium Dissectum
- Van Wagenen BC, Huddleston J, Cardellina JH -
NATURAL PRODUCTS,1988 51(1)136-141. IS ref.

Macrocyclic pyrrolizidine alkaloids from Senecio anonymus. Separation of a
complex alkaloid extract using droplet counter-current chromatography
- Zalkow LH, Asibal CF, Blinski JA, et al. - j NATURAL PRODUCTS, 1988 51(4)690-702

Isolation and structure of the cytostatic lignan glycoside phyllanthostatin A
- Pettit GR, Schaufelberger DE - NATURAL PRODUCts,1988 51(6)1104-1112. 18 ref.

I 0-hydroxy-Nb-methyl-corynantheol, a new quarternary alkaloid from the stem
bark of Strychnos usambarensis
- Quetin-Leclerq, Angenot L - PHYTOCI-4EMISTRY, 27(6)1923-1926. 7 ref.

Isolation and purification of panarine A. Alkaloid from a Venezuelan curare
- Quetin-Leclercq J, Angenot L Dupont L, et al. -
PHYTOCHEMISTRY, 1988 27(12)4002-4004. 7 ref.

Principles and instrumentation of countercurrent chromatography
- Ito Y - pp 79-442 in: Countercurrent Chromatography: Theory & Practice,ed.by NB Mandava & Ito Y,Dekker,New York, 1988
Centrifugal partition chromatography (CPC) as a useful method for determination of partition coefficient between octanol and water
- Terada H, Kosuge Y, Nakaya N. et al. -

Coloradocin, an antibiotic from a new actinoplanes. II. Identity with luminamicin and elucidation of structure
- Rasmussen RR, Scherr MH, Whittern DN, et al. -
ANTIBIOTICS, 1987 XL( 10 ) 1383-1393. 20 ref.

Counter current chromatography - applications to the separation of biopolymers, organelles and cells using either aqueous-organic or aqueous- aqueous phase systems
- Sutherland IA, Heywood-Waddington D -

Correlation of hydrophobic parameters of organic compounds determined by centrifugal partition chromatography with partition coefficients between octanol and water
- Terada H, Kosuge Y, Murayama W, et al. -
CHROMATOGRAPHY, 1987 400 343-351. 20 ref.

Apocarotenoids from Cochlospermum tinctorium
- Diallo B, Vanhaelen M -
PHYTOCHEMISTRY, 1987 26(5)1491-1492. 14 ref.
Countercurrent chromatography in the fractionation of natural products
- Martin DG, Biles C, Peltonen RE -
AMERICAN LABORATORY,1986 1821-22,24-26. 23 ref.

Separation of the components of commercial digitonin using high-performance liquid chromatography and centrifugal countercurrent chromatography. Identification of the products by Californium-252 plasma mass spectrometry
- Yang YM, Lloyd HA, Panneli LK, et al. -

High-speed counter-current chromatography
- Ito Y -

Preparative resolution of an actinomycin complex by countercurrent chromatography in the Ito coil planet centrifuge
- Martin DG, Peltonen RE, Nielsen JW -
ANTIBIOTICS, 1986 39(5)721-723

Benzanthrins A & B, a new class of quinone antibiotics. II. Isolation, elucidation of structure and potential antitumor activity
- Rasmussen RR, Nuss ME, Scherr MH, et al. - A
NTIBIOTICS,1986 39(11)1515-1626. 17 ref.

Isolation of artemisinin (ginghaosu) and its separation from artemisitene ising the
Ito multilayer coil separator-extractor and isolation of arteanniun B
- Acton N, Klayman DL Rollman IJ, et al. -
Fractionation of condensed tannins by counter-current chromatography
- Putman U, Butler LG -
CHROMATOGRAPHY,1985 318 85-93. 20 ref.

Large scale preparative counter-current chromatography with a coil planet centrifuge
- Sandlin JL, Ito Y -

Use of coil planet centrifuge in the isolation of antibiotics
- Brill GM, McAlpine JB, Hochlowski JE -
LIQUID CHROMATOGRAPHY,1985 82259-2280. 10 ref.
Experimental observation of the hydrodynamic behavior of solvent systems in high-speed countercurrent chromatography. I. Hydrodynamic distribution of two solvent phases in a helical column subjected to two types of synchronous planetary motion
- Ito '5' - CHROMATOGRAPHY, 1984 301 371 +

Horizontal flow-through coil planet centrifuge: some practical applications of countercurrent chromatography
- Zhang TY -

Massive Process Cost Savings.

No Flash/SFC/HPLC Phase to purchase.

No replacement solvent to purchase.

Appropriate solvent choice, allows very
low cost recycling of your CPC solvents.

After instrument purchase, your main
running cost will only be Electricity.


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Laboratory Scale Quattro CPC & CCC Prices

Quattro QuikPrep™ / LabPrep™ CPC & CCC units: 250 to 2000ml total instrument volumes. Options of multiple volumes per instrument from 30ml. Price range £27,500 to £87,500.


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Scaling-Up Your Application?

Scale-up to multiple 10’s tonnes per annum possible. For multiple 100’s tonnes per annum, please phone or email

Pilot & Process Scale Quattro CPC Prices

4.8 litres Quattro PilotPrep™ CPC £136,000


1 x 12 litres Quattro ProcessPrep™ CPC £187,000

2 x 12 litres Quattro ProcessPrep™ CPC £340,000

4 x 12 litres Quattro ProcessPrep™ CPC £625,500

1 x 50 litres Quattro ProcessPrep™ CPC £650,000


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To the above pricing for scale-up, must be added customised Ancillary Instrumentation:- Pumps, Detector, Fraction Collection, Control Computer Etc., to match your needs and budget. Or provide your own existing or new ancillary instrumentation.                    

Please consider visiting our NEW DEMONSTRATION LABORATORY near Newquay Airport, only 1 hour flight from London Gatwick, and with good connections to many National & International Airports.

Please consider requesting Dr Leslie Brown visiting you to discuss your larger scale process needs.

If you are interested in collaborative research, please suggest a topic. We are particularly interested in high pressure SFE / SFC / SFC-MS / L-LC direct to MS applications, but have a general interest in many subjects from trace to process quantities.

Click on picture on left for "Quattro CCC™ NEEM Research" slide show scientific presentation
Click on picture on left for "Quattro CCC™ WINE Research" slide show scientific presentation
Click for General applications slides (extra notes in comments sections)
Click for Glycoside applications slides
Click for Anti-Oxidant Research application slides
Click for comparison Solid-Liquid and Liquid-Liquid Chromatography

1Quattro CCC & CPC available for demonstration at our New Research Laboratory, near Newquay Airport, Cornwall, England

2) Multiple Quattro Process CPC installed for MEDICINAL CANNABIOID PURIFICATIONS, both Quattro PilotPrep CPC and multiple installations of Quattro ProcessPrep CPC

3) Most Quattro ProcessPrep CPC installations for MEDICINAL CANNABINOID PROCESSES. MARIJUANA EXTRACTS (only to licensed THC producers) and HEMP PROCESSORS (for CBD and minor cannabinoid purification, and THC removal from broad spectrum Hemp Extracts).